Single-cell sequencing of immune cells after marathon and symptom-limited cardiopulmonary exercise

Single-cell sequencing of immune cells after marathon and symptom-limited cardiopulmonary exercise

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Summary: Vigorous-intensity leisure-time physical activity, such as marathon, has become increasingly popular, but its effect on immune functions and health is poorly understood.Here, we performed scRNA-seq analysis of peripheral blood mononuclear cells (PBMCs) after a bout of symptom-limited cardiopulmonary exercise (CPX) test or marathon.Time-series single-cell analysis revealed the detailed series of landscapes of immune cells in response to short click here and long vigorous-intensity activities.Reduction of effective T cells was observed with the cell migration and motility pathways enriched in circulation following marathon.

Baseline values of PBMCs abundance were reached around 1 h after CPX and 24 h following marathon, but longer time was required for expression recovery of cytotoxicity genes.The ratio of effector/naive T cells was found to change uniformly lolasalinas.com among the participants and could serve as a better indicator for exercise intensity than the CD4+/CD8+ T cell ratio.Moreover, we identified time-dependent monocyte state transitions after marathon.